日本ゼオライト学会　刊行物 Publication of Japan Zeolite Association

We have succeeded in assembling a highly durable membrane capable of high-density accumulation to have developed encapsulation technologies of enzymes into nanoporous membrane by controlling their pore sizes and conditions, and a novel detection method, based on the biosensors composed of an immobilized enzymes in the mesoporous silica membranes, an electrochemical mediator and an electrochemical cell, using the enzyme, i. e. formaldehyde dehydrogenase or acetylcholinesterase. Those biosensors show rapid response and high sensitivity in aqueous solution. Furthermore, those sensors show high selectivity, reusability and remarkable storage stability, indicating enzymes remains in highly ordered structure in those mesoporous silica membrane. These results indicate that the mesoporous silica materials can provide favorable methods for enzyme immobilization on the electrode and then are useful for electrochemical biosensors with high performance. Additionally, choosing proper enzymes for the purposes and optimizing the pore size of mesoporous silica membrane, the technique is generally applicable to the detection of various other substrates and contributes to the development of small-sized and high-performance sensing device.